The aim of this project is to construct and evaluate an adenovirus based vaccine for Campylobacter jejuni; vaccines based upon the genes encoding immunogenic FlaA and Omp18 antigens and a multi epitope vaccine based upon the C. jejuni FlaA immunogenic epitopes and Omp18 gene (sequences will be based upon published gene sequences) will be constructed using the AdHu5 vector platform and tested in chickens. The AdHu5 vector is a commercially available vector that has been shown to be safe and efficacious for vaccine development. The viral vector based vaccine will be propagated in cell culture and the vaccine will be purified using standard techniques. The vaccine will be tested in cell culture to confirm expression of vaccine antigens (antigen expression will be confirmed using Western blots). The first dose of vaccine will be delivered to 1 day to 1 week old chicks (oral routeadministered in water) or into 18 day old eggs using well established in ovo vaccine delivery techniques whereby a vaccine can be delivered to a developing embryo in the egg. All chicks will be boosted. The boost will be delivered orally at different time points (following the first dose of vaccine (prime) or post hatch for chicks that received the in ovo vaccine; (vaccine doses tested will range from 103 to 108 virus particlesboth prime and boost). The efficacy of the vaccine will be determined by bleeding the chicks at various time points and determining antibody titres (IgG) to FlaA and Omp18 antigens. Vaccinated chickens will be challenged (via oral route) with a clinical isolate of C. jejuni at 14 to 21 days of age (minimum of 7 days after vaccination) and campylobacter colonisation determined at weekly time points for up to 6 weeks post challenge (faecal C. jejuni counts and isolation of C. jejuni from the intestinal tract of euthanised chickens).
University of South Australia
To construct an adenovirus based vaccine for Campylobacter jejuni; To evaluate the effectiveness of the vaccine in preventing colonisation of chickens with C jejuni.
Project Start Date
Monday, March 1, 2010
Project Completion Date
Friday, February 28, 2014
Journal Articles From Project
Promoting and maintaining good health
CME-Improving Food Safety of Australian Chicken Meat