Colic or abdominal pain is one of the most common and potentially devastating conditions encountered by equine veterinarians. The most severe triggers of colic can be caused by impaired intestinal perfusion resulting in inflammation that is characterized by oxidative stress and increased production of isoprostanes. In some cases, surgical intervention may ultimately be curative but may be delayed because of a lack of accurate diagnostic tests. The delay of surgery may lead to even more severe illness. In other cases of colic, surgery may be unnecessary, and the onset of postoperative complications can be devastating and expensive. For this reason, reliable indicators (biomarkers) of intestinal injury are needed to objectively predict the need for exploratory abdominal surgery. Indicators of oxidative stress, such as isoprostanes have been used to identify inflammation but have not been assessed within abdominal fluid samples and may reflect the severity of abdominal perfusion impairment. We believe that isoprostanes can serve as a biomarker (indicator) for surgical intervention in horses with colic and serve potentially as a future therapeutic target to minimize bowel damage secondary to perfusion impairment and to prevent potential sequala’s as reperfusion injury and laminitis, the second highest mortality factor of horses after colic.
The University of Adelaide
The major objectives are: The purpose of this study is to determine peritoneal fluid concentrations of the isoprostane PGF2a (isoPGF2a) and its metabolite (isoPGF2aM) in a population of normal horses and horses with colic. The hypothesis of this study is that peritoneal fluid isoprostane levels are a sensitive biomarker predicting the need for surgery and may be used as a therapeutic target. The major objectives are: 1) to determine the feasibility of using peritoneal fluid isoprostane concentrations as a biomarker for the presence of gastrointestinal disease requiring surgical intervention in horses with colic; 2) to assess the viability of using peritoneal fluid isoprostane concentrations as a prognosticator for horses with colic. The data from this study will lead to a broader multi centre research project that will investigate the feasibility to use isoprostanes as a therapeutic target to prevent potential sequala’s as reperfusion injury and laminitis, the second highest mortality factor of horses after colic.
Project Start Date
Wednesday, July 1, 2020
Project Completion Date
Saturday, December 31, 2022
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