The development of horse embryonic stem cells (eESCs)

Summary

unknown

Program

Thoroughbred Horses

Research Organisation

Monash University

Objective Summary

The project aims to develop pluripotential equine embryonic stem cells (eESCs) from morulae blastocysts and by separation and culture of the ICM of the horse blastocyst. This method has been used to derive mouse and primate ESCs that are immortal and capable of differentiation into all the cells of the organism. In addition we propose to derive equine specific eESCs (ESESCs) by the insertion of horse somatic cell nuclei and mitochondria into enucleated mouse or rabbit eggs to isolate eESCs from xenogenic embryos. eESCs cell will be characterised by methods used for mice primate and humans.