We will quantify the threats to the industry associated with interspecific matings by the following experiments: 1) In Cairns we will perform reciprocal artificial inseminations of A. cerana and A. mellifera. We will study the eggs of the queens to determine if there is embryogenesis. We will allow some brood to emerge in an incubator to quantify the proportion of offspring that are haploid males, inviable hybrids and thelytokous females. We will use microsatellites to confirm the maternity and (lack of) paternity in the offspring. 2) In the Solomon Islands where there are extremely dense populations of the Java strain of A. cerana we will determine the drone flight time of the males of both species to see if there is overlap. If logistically feasible we will determine the location of DCAs of A. mellifera and A cerana. Finally, we will examine the offspring of A. mellifera queens that we allow to naturally mate with A. cerana males.
The University of Sydney
To determine if interspecific matings occur between the Java strain of A. cerana and A. mellifera. To determine the consequences of such matings on brood viability. To determine if interspecfic matings can induce thelytoky. To determine if there is overlap in the drone flight time between A. mellifera and the Java strain of A. cerana. To determine if A. cerana and A. mellifera drones can be caught in the same area, indicating overlap in their mating leks (sometimes known as 'drone congregation areas').
Project Start Date
Tuesday, May 29, 2012
Project Completion Date
Thursday, May 15, 2014
Journal Articles From Project
HBE-Improve understanding of nutrition best practice and disease interaction