The overarching goal of this study is twofold. Firstly, an extensive survey of MLresistance is proposed for the southeast QLD, Hunter Valley and North QLD regions to document MLefficacy and prevalence of resistance. Secondly, the project aims to develop and/or evaluate a number of in vitro assays to identify differences in susceptibility of equine worm populations to ML anthelmintics. Of primary importance is the development of an assay for use in determining MLresistance within P. equorum isolates. Two assays which have been previously described as applicable to Trichostrongylid parasites of sheep; the larval development assay and the larval migration assay, will be evaluated for their ability to detect variation in ML susceptibility between cyathostomin populations.
The University of Queensland
The objectives of the project are: 1. To provide data on MLresistance in P. equorum and cyathostomins at several regions in eastern Australia. 2. To develop in vitro assays that can be used to effectively identify variations in susceptibility to ML drugs in P. equorum. 3. To develop in vitro assays that can be used to effectively identify variations in susceptibility to ML drugs in cyathostomin populations, 4. To investigate the practicability of use of the Reverse Line Blot Hybridization technique (able to unequivocally detect and identify 13 cyathostomin species) in resistance studies.
Project Start Date
Tuesday, November 27, 2012
Project Completion Date
Saturday, January 31, 2015
Journal Articles From Project
Frontier technologies for building and transforming Australian industries
Adoption of R&D
HOR-Reduce the incidence and impact of diseases and parasites in horses