Differential typing of Campylobacter

Summary

The outcome of this project will be a DNA based typing scheme that allows the allocation of strains of Campylobacter jejuni/coli to types that are host specific and/or non host specific.

Program

Chicken Meat

Research Organisation

OLD ABN-The State Of Queensland Acting Through The Department of Employment, Economic Development and Innovation

Objective Summary

1. Establish the technique of Single Nucleotide Polymorphism (SNP) analysis for typing of C. jejuin/coli. 2. Extend the current Campylobacter collection to include poultry isolates from other research groups and industry and to obtain nonpoultry isolates from sources such as diary cattle pets and humans. 3. Establish SNP types of the extended Campylobacter collection. 4. Compare and contrast the SNP types of the poultry C. jejuni with the nonpoultry types (including human isolates from OzFood that have already been examined by SNP typing by the Qld node of the CRC for Diagnostics) 5. Compare and contrast the capacity of SNP typing with pulsed field gel electrophoresis (PFGE) and flaA typing. 6. Establish an ongoing typing technique that will function as a national reference service on a user pays basis.