This literature review, funded by the AgriFutures™ Honey Bee and Pollination Program, aims to identify and review current and new analytical techniques for honey analysis, and the limitations of these techniques. This includes the determination of limitations of different analytical techniques, given the particular floral characteristics of Australian derived honey, as well as the effect of supplementary feeding on analytical results. A review of national and international regulatory policies and guidelines on honey testing and the determination of honey chemistry was also completed.