Summary

This project will build on the recently established Multi-Locus Sequence Typing (MLST) scheme developed by this team (using RIRDC funding) for Pasteurella multocida. The rapid techniques (Single Nucleotide Polymorphisms – SNPs and High Resolution Melt Analysis – HRM), used by this research team for Campylobacter typing, will be developed for the rapid, cost effective first level screening of P. multocida isolates. The new rapid methods will predict MLST results and reduce the need for full sequencing (seven genes with 500 bases per gene). The results can then be used to select isolates for full MLST and/or serotyping, improving on the current situation where all isolates have to be fully serotyped or fully MLST typed (both expensive and time consuming methods). This project will deliver be a more rapid and cost-effective typing scheme for P. multocida.

Program

Chicken Meat

Research Organisation

The University of Queensland

Objective Summary

A) Identify SNPs that are mathematically shown to provide maximum predictive power for all seven genes in the P. multocida MLST scheme.
B) Validate SNPs by comparing results from SNP analysis with full MLST using sets of epidemiologically related and un-related strains of P. multocida.
C) Develop HRM methodology to predict MLST results using a set of strains that are fully MLST typed.
D) Validate HRM methodology for predicting MLST results using sets of epidemiologically related and un-related strains of P. multocida.
E) Provide on-going typing service – including MLST and serotyping – for free to industry during the life of the project.